Webb3 jan. 2012 · Phosphorylated Tie2 in cells treated with VEGF, PMA or TNFα for 0, 0.5 or 24 h and activated with Ang1 was detected by immunoblotting in at least three independent experiments and quantitated by densitometric scanning. Data are means and SEM of phospho-Tie2 normalized to that in Ang1-activated control cells within each experiment. WebbMechanistic Effects of Autophosphorylation on Receptor Tyrosine Kinase Catalysis: Enzymatic Characterization of Tie2 and Phospho-Tie2 Brion W. Murray,* Ellen S. Padrique, Chris Pinko, and Michele A. McTigue Pfizer …

Anti-phospho-TIE2 (pTyr 992 ) antibody produced in rabbit - Sigma …

WebbTie-2 signaling is activated by Angiopoietins-1 and -4, while it can be either activated or inhibited by Angiopoietins-2 and -3. Tie-2 plays an important role in maintaining vascular … WebbTie-2, also known as Tek, is a transmembrane receptor tyrosine kinase (RTK) that functions as a receptor for Angiopoietin family proteins. It is expressed by embryonic and adult … tatrapeko svit kontakt

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Webb1 jan. 2009 · In addition, the phosphorylated forms of Akt and Erk1/2 were measured to confirm activation of signaling pathways downstream of Tie2. While HPTPβ knockdown did not alter basal levels of Tie2 phosphorylation, Ang-1-induced Tie2 phosphorylation was significantly increased 2.52-fold, P < 0.001 (Fig. 3a). Webb28 aug. 2001 · Autophosphorylation of the angiogenic receptor tyrosine kinase Tie2 was studied in terms of the autophosphorylation sites, sequence of phosphorylation at these sites, kinetic effects, and mechanistic consequences. Isoelectric focusing electrophoresis and mass spectrometric analysis of a Tie2… View on PubMed doi.org Save to Library … Webbinhibition of the phospho-TIE2 signal, which is subsequently quantified by Sandwich-ELISA technique. The assay is validated based on known inhibitors of TIE2 kinase activity (see Fig. 1). Figure 1: Assay validation. Sorafenib is a potent inhibitor of TIE2 phosphorylation with highly reproducible IC50 values. The graph shows a representative result. tatranske plesa