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FLP-FRT recombination - Wikipedia
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WebFrom a technique standpoint, the Flp-FRT system for the generation of genetic mosaics has been a principle tool. It has broadly been used in the discovery of Hippo pathway members in mutagenesis screens, in the analysis of target gene expression, and in genetic epistasis. In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism's DNA under controlled conditions in vivo. It is analogous to Cre-lox recombination but involves the recombination of sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp) derived from the 2 µ plasmid of baker's yeast Saccha… WebUsing suitable genetic crosses to combine the FLP/FRT and Gal4/UAS system, we generated stocks where bi-Gal4 can drive expression of FLP within its expression domain, and trigger homologous recombination at the site of the FRT cassettes to generate homozygous loss-of-function clones (Fig. 2). We established fly stocks which allow generation of ... skills session red cross