Phellsgate 2
WebAug 1, 2015 · C12N15/825 Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic... WebThe pHELLSGATE vectors contain two recombination cassettes consisting of either attP1-ccdB-attP2 or attR1-ccdB-attR2 in an inverted repeat configuration, such that when gene …
Phellsgate 2
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WebJan 16, 2014 · Recombinations reaction between the pDONOR221-attB-PHYA1 and the pHellsgate-8 vector with 35S promoter 11 were conducted in a total volume of 10 μl, with 2 μl LR clonase buffer (Invitrogen), 2 ... WebBriefly, the partial fragments of FaSAUR1 and FaSAUR2 (Supplementary Figure S5) were ligated into pHELLSGATE 2 vector (Shi et al., 2024) using BP Clonase (Invitrogen, MA, USA) to construct 35Spro...
WebIn this paper we describe two improved pHellsgate vectors that facilitate rapid generation of hpRNA-encoding constructs. pHellsgate 4 allows the production of an hpRNA construct in … WebpHELLSGATE载体价格10000元，pHELLSGATE载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性壮观霉素或链霉素，质粒大小为18691BP，测序引物见 …
Web2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g. 5.Store the plasmid at -20 ℃. pHELLSGATE 8 vector Sequence Copy Sequence WebSep 19, 2016 · The plasmids pHellsgate-Bc-DCL1/2 and pHellsgate-Bc+Vd-DCLs were constructed following the methods outlined for the Hellsgate8.0 system 51. The Bc-DCL1/2 RNAi fragment was obtained by integrating Bc-DCL1 (252 bp) and Bc-DCL2 (238 bp) via overlapping PCR.
WebpHELLSGATE 12 High-throughput binary Gateway® vector with two introns, for silencing genes in plants using intron-containing hairpin RNA. Open in SnapGene Try SnapGene for …
WebOverwatch 2 Elden Ring Genshin Impact GTA Reviews Giveaways Videos Free Halo x Portal Shooter Coming to Consoles In August. Share Facebook Twitter Reddit WhatsApp Copy … guthrie county court recordsWeb4 beds, 3 baths, 2278 sq. ft. house located at 2302 Fieldgate Dr, Pittsburg, CA 94565. View sales history, tax history, home value estimates, and overhead views. APN ... guthrie county courthouse hoursWebpHELLSGATE is an efficient, high-throughput and effective plant gene silencing vector. Vector Type. Gene Silencing Vector, Gateway Vector, Plant Dual Expression Vector ... Powder; Solution Size. 2 μg; 10 μL . High Copy/Low Copy. High Copy . Prokaryotic Resistance. Spectinomycin . Length. 18691 bp . Selectable Marker. guthrie county fsa officeWebAug 1, 2003 · The pHELLSGATE vectors A high-throughout cloning system to generate hpRNA constructs requires a vector, such as pHANNIBAL, to give directional insertion of a gene fragment into an inverted repeat conformation separated by an intron. box red velvet cheesecakeWebBioz Stars score, Techniques, Protocol Conditions and more for Binary Vector Phellsgate 2, supplied by Thermo Fisher. Data for Binary Vector Phellsgate 2 gathered from related … guthrie county electricWebThe pHELLSGATE vectors contain two recombination cassettes consisting of either attP1-ccdB-attP2 or attR1-ccdB-attR2 in an inverted repeat configuration, such that when gene fragments flanked by the appropriate att sites are recombined with the vector, an ihpRNA-encoding construct is produced ( Fig. 3 ). guthrie county hospital clinic in panoraWebSep 28, 2010 · pHELLSGATE is an RNAi vector used to validate gene function in plants. In the present study, recombinant pHELLSGATE vector was constructed with amplicons of the gene serine threonine protein kinase (STPK) cloned from Piper colubrinum. box red velvet cake mix recipe