Web30 dec. 2015 · After 30 min incubation, mitochondria were fixed and stained with MitoTracker (blue), while ceramide and Bax were localized using anti-ceramide IgM (red) or anti-Bax IgG (green), respectively. Control IgM and IgG did not yield detectable signals (not shown). These data represent 1 of 3 similar studies. WebMitoTracker® Deep Red FM is a far red-fluorescent dye (abs/em ∼644/665 nm) that stains mitochondria in live cells and can be used for mitochondrial localization. The dye is well-retained after aldehyde fixation and even …

MitoTracker® Green FM Cell Signaling Technology

WebThermo Fisher dapi Dapi, supplied by Thermo Fisher, used in various techniques. Bioz … WebFigure 5: Toxic effect of Antimycin A on mitochondria. (Top) Overlay of Hoechst (blue) … rawlsian ideal

Mitochondrial and plastidial COG0354 proteins have folate …

Web7 jun. 2016 · D: MitoTracker red CMXRos staining of immortalized kidney epithelia from WT and jck animals. jck cells treated with 30 μM C2-13 for 60 min, untreated WT cells, and untreated jck cells were stained with 250 nM Mitotracker red CMXRos for 30 min, fixed in 4% paraformaldehyde, and imaged. Red shows MitoTracker; blue shows DAPI. Web(C) Phase-contrast and MitoTracker fluorescence images of hMADS cells that were treated or not with 100 nM ANP, as described in Methods. Mitotracker is identified by the orange-red color. DAPI-stained nuclei are blue (original magnification, ×20). (D) mtDNA content was quantified by qPCR, as described in Methods. Bars represent mean ± SEM. WebThermo Fisher mitotracker blue Mitotracker Blue, supplied by Thermo Fisher, used in … simple hemp cream