WebHowever, the functional role and molecular mechanisms of FTO in pancreatic beta cells is still unclear. In this study, we aim to investigate the role of FTO in the pancreatic beta cells using a new pancreatic beta cell line tool for beta cell function called GRINCH cells (Glucose-Responsive Insulin-secreting C-peptide- modified Human-proinsulin). WebSep 5, 2024 · GRINCH cells were developed to study proinsulin trafficking, processing and secretion from pancreatic β-cells. The superfolder GFP inserted in human …
Distinct insulin granule subpopulations implicated in the ... - eLife
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WebMar 1, 2024 · GRINCH cells displayed neuronal markers, and their functional activity could be demonstrated by electrophysiological recordings. In an application of GRINCH neurons, the brain-derived neurotrophic factor (BDNF)–mediated activation of tropomyosin receptor kinase (TrkB) was investigated as a promising drug target to treat synaptic dysfunctions. WebMar 1, 2024 · To assess neuronal marker expression on the protein level, the GRINCH neurons were analyzed by immunofluorescence staining (Fig. 4A–C).Beta-3-tubulin (Tuj-1), the TUBB3 encoded marker expressed on neurons but not on glial cells, 14 was found to be expressed on a vast majority of the cells described here, with its typical staining pattern … WebMar 22, 2024 · Because cells contain a reservoir of long-lived mature granules that could predate and thus persist long after siRNA-mediated transfection, we decided to transiently transfect GRINCH cells to newly express a fluorescent-tagged secretory protein (NPY-mCherry, a very good marker for insulin granules; Figure S3). instant coffee in individual packets